Intermediate Filament Cytoskeleton

Intermediate Filament Cytoskeleton PDF
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Author: M. Bishr Omary
Publisher: Gulf Professional Publishing
ISBN: 9780125641739
Size: 67.59 MB
Format: PDF, Docs
Category : Medical
Languages : en
Pages : 886
View: 4796

Intermediate filaments are a large family of proteins that are the cytoskeletal elements involved in a number of skin, liver, neuromuscular, cardiac, eye and hair diseases. Intermediate filament genes are regulated in a tissue-and cell type-specific manner and their polymerized protein products protects the cells and tissue they are part of against a variety of mechanical and nonmechanical stresses. This book provides a comprehensive resource of methodology essentials, describing a variety of essential tools and assays for studying intermediate filaments. The book provides user-friendly advice and protocols covering all aspects of intermediate filaments including protein isolation and structure, protein and gene regulation, relationship to disease and apoptosis, and associated proteins. Both mammalian and non-mammalian systems and animal models are covered, making this book a must-have for any investigator wishing to study IF genes or their protein products. * Covers intermediate filaments from crystallography, protein chemistry, cell and molecular biology, microrheology, gene regulation, to animal models and human disease * Practical and user-friendly with detailed "how-to-protocols and "tricks of the trade" * Includes detailed tables of useful reagents, vendors and web links


Intermediate Filament Cytoskeleton

Intermediate Filament Cytoskeleton PDF
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Author:
Publisher: Academic Press
ISBN: 9780125641739
Size: 45.34 MB
Format: PDF, Kindle
Category : Science
Languages : en
Pages : 855
View: 5752

Intermediate filaments are a large family of proteins that are the cytoskeletal elements involved in a number of skin, liver, neuromuscular, cardiac, eye and hair diseases. Intermediate filament genes are regulated in a tissue-and cell type-specific manner and their polymerized protein products protects the cells and tissue they are part of against a variety of mechanical and nonmechanical stresses. This book provides a comprehensive resource of methodology essentials, describing a variety of essential tools and assays for studying intermediate filaments. The book provides user-friendly advice and protocols covering all aspects of intermediate filaments including protein isolation and structure, protein and gene regulation, relationship to disease and apoptosis, and associated proteins. Both mammalian and non-mammalian systems and animal models are covered, making this book a must-have for any investigator wishing to study IF genes or their protein products. * Covers intermediate filaments from crystallography, protein chemistry, cell and molecular biology, microrheology, gene regulation, to animal models and human disease * Practical and user-friendly with detailed "how-to-protocols and "tricks of the trade" * Includes detailed tables of useful reagents, vendors and web links


Effects Of Cell Stretch On The Keratin Intermediate Filament Cytoskeleton In A549 Lung Cells

Effects of Cell Stretch on the Keratin Intermediate Filament Cytoskeleton in A549 Lung Cells PDF
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Author: Anngrit Maria Lutz
Publisher:
ISBN:
Size: 67.46 MB
Format: PDF, ePub, Mobi
Category :
Languages : en
Pages :
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The Cytoskeleton In Health And Disease

The Cytoskeleton in Health and Disease PDF
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Author: Heide Schatten
Publisher: Springer
ISBN: 1493929046
Size: 67.94 MB
Format: PDF, ePub
Category : Science
Languages : en
Pages : 395
View: 4882

This volume addresses the structural and functional roles of the cytoskeleton and its dysfunctions which often lead to disease. It provides thorough discussion of microtubules, microfilaments, intermediate filaments, and cytoskeletal functions and dysfunctions in different organ systems. Comprehensive yet concise. The Cytoskeleton In Health And Disease presents cutting-edge discoveries balanced with background information and highlights the new aspects of the research and its impact on the design of new strategies or the identification of new targets for therapeutic intervention. There is a significant need for a book on this topic, as interest in the cytoskeleton continues to grow as causes and cures for cytoskeletal diseases are further explored in biomedical research. This book is essential reading for scientists, students, and teachers interested in expanding their knowledge related to the cytoskeleton. New researchers entering the field will find classic and well as contemporary information not easily found in the current literature or internet resources.


Intermediate Filaments

Intermediate Filaments PDF
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Author: Harald Herrmann
Publisher: Springer Science & Business Media
ISBN: 9780306458545
Size: 15.38 MB
Format: PDF, ePub
Category : Science
Languages : en
Pages : 622
View: 2300

This text looks at subcellular chemistry, particularly at intermediate filaments. Topics include fish intermediate filament proteins in structure, evolution, and function, and lessons from keratin transgenic and knockout mice.


Intermediate Filaments

Intermediate Filaments PDF
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Author: P. Traub
Publisher: Springer Science & Business Media
ISBN: 3642702309
Size: 26.15 MB
Format: PDF, ePub, Docs
Category : Science
Languages : en
Pages : 266
View: 1811

Research on cytoskeletal elements of eukaryotic cells has been expand ing explosively during the past 5 to 10 years. Due largely to the employment of electron and immunofluorescent microscopy, significant results have been obtained which have provided interesting new insights into the dynamics of nucleated cells at the structural, physiological, as well as developmental levels. While a substantial amount of knowledge has accumulated on the function of microfilaments and microtubules, the roles of the third major class of cytoskeletal structures in vertebrate cells, the intermediate filaments, have largely resisted clarification. The investigation of cultured cells and of tissues from various developmental stages has furnished a host of information on the inter-and intracellular distribution of the different types of intermediate filaments and led to the contention that they have a structural and organizing function in the cytoplasm of vertebrate cells. However, the results of recent experimen that vertebrate cells can function perfectly in the tation have shown complete absence of cytoplasmically extended intermediate filament meshworks. It is legitimate to suppose, therefore, that their function in vertebrate cells is much more subtle and complex than generally presumed. Our interest in the structure and function of intermediate filament proteins was initiated approximately 7 years ago while working on the regulation of macromolecular synthesis in picornavirus-infected mam malian cells. In attempts to demonstrate virus-induced changes in the nuclear protein components of the host cells, the nonionic detergent extraction method was used to purify nuclei.


Intermediate Filament Cytoskeleton

Intermediate filament cytoskeleton PDF
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Author:
Publisher: Academic Press
ISBN: 9780124802834
Size: 29.31 MB
Format: PDF
Category :
Languages : en
Pages : 886
View: 3110

The chapters in CYTOMETRY MCB volumes, including this 4th Edition, provide comprehensive description of particular cytometric methods and review their applications. Some chapters also describe new instrumentation and provide fundamental information on use of new fluorescent probes and on data analysis. Although the term "edition" suggests the update of earlier volumes, in fact, nearly all chapters of the 4th Edition are devoted to new topics. The authors were invited to present not only technical protocols, such as available in other methodology books that specialize in the protocol format, but also to discuss the aspects of the methodology that generally are not included in the protocols. Many chapters, thus, present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical setting, common traps and pitfalls, problems with data interpretation, comparison with alternative assays, choice of the optimal assay, etc. Some chapters review applications of cytometry and complementary methodologies to particular biological problems or clinical tasks. The 34 chapters presented in CYTOMETRY 4th Edition cover a wide range of diverse topics. Several chapters describe progress in technology of fluorescence measurement. The novel phenomenon of the surface-plasmon coupled emission (SPCE) presented in one of these chapters, in combination with nanophotonic technology, is expected to open entirely new possibilities for ultra-sensitive detection of fluorescence, likely from single fluorochrome molecules. The chapter on cytometry of fluorescence resonance energy transfer (FRET) describes the theoretical foundations- and uncovers further analytical possibilities- of this methodology. The chapter on fluorescent proteins is an exhaustive review of accomplishments and possibilities offered in this rapidly expanding field. The critical assessment of quantitative analytical capabilities of confocal microscopy, optimization of emission optics and further progress in development of laser scanning cytometry instrumentation are the topics of other chapters focused on methodology of fluorescence measurement. Another group of chapters describe variety of cytometric methods and their applications in studies of cell death, particularly by mode of apoptosis, mechanism of antitumor drug action, and DNA damage. The rapidly growing application of cytometry in phytoplankton is also assessed in great detail. The chapters on biohazard sorting and data analysis guidelines will be of interest to a variety of researchers in different fields. Cell immunophenotyping represents the most common application of cytometry, particularly in clinical setting. It is not surprising, therefore, that over a third of all chapters of this volume are devoted to methods and applications of multi-color immunophenotyping and probing immune functions of lymphocytes and phagocytes. The field of cytogenetics and molecular genetics is represented by several chapters. Presented are the methods for telomere length measurement and genomic array technology as well as multiplex amplifiable probe hybridization (MAPH) and multiplex ligation -dependent probe amplification (MLPA), the latter to probe the copy number changes (deletions, multiplications) in genomic DNA. A very exhaustive is the chapter describing the use of subtelometric probes in studies of mental retardation. In tradition with the earlier CYTOMETRY editions, the chapters were prepared by the researchers who either developed the described methods, contributed to their modification, or found new applications and have extensive experience in their use. The list of authors, thus, is a continuation of "Who's Who" directory in the field of cytometry. * Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays * Discusses many new assays developed since the previous edition * Presents recent developments in cytometric intrumentation/technology.


Cytoskeleton Of The Nervous System

Cytoskeleton of the Nervous System PDF
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Author: Ralph A. Nixon
Publisher: Springer Science & Business Media
ISBN: 9781441967879
Size: 58.84 MB
Format: PDF, Docs
Category : Medical
Languages : en
Pages : 774
View: 2463

This monograph begins with a general description of the cytoskeleton in axonal development and pathology and then moves to more detailed descriptions of particular components, including microtubules and associated proteins, neurofilaments and interacting proteins, actin and its binding proteins, and glial fibrillary acidic protein. The later chapters focus on the functional significance of the neuronal cytoskeleton in axonal transport and its regulation in health and disease states. The Cytoskeleton of the Nervous System will encourage further development of unifying principles and stimulate new conceptual and technical approaches toward a better understanding of cytoskeleton functions in health and disease.


Cumulated Index Medicus

Cumulated Index Medicus PDF
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Author:
Publisher:
ISBN:
Size: 57.36 MB
Format: PDF, ePub, Docs
Category : Medicine
Languages : en
Pages :
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Guidebook To The Cytoskeletal And Motor Proteins

Guidebook to the Cytoskeletal and Motor Proteins PDF
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Author: Thomas Kreis
Publisher: Sambrook and Tooze Publication at Oxford University Press
ISBN: 9780198599579
Size: 41.86 MB
Format: PDF, Docs
Category : Medical
Languages : en
Pages : 551
View: 3864

Since the first edition, many new and important discoveries have been made in this expanding area of research. This updated edition details all that is currently known about the proteins involved in the microfilament, microtubule and intermediate filament systems in cells and their roles intension resistance and motor function. Biochemists will find this book particularly useful as will physiologists and biologists in fields in which these proteins are involved, such as neuromuscular research.